RAC: Repository of Antibiotic resistance Cassettes
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چکیده
منابع مشابه
RAC: Repository of Antibiotic resistance Cassettes
Antibiotic resistance in bacteria is often due to acquisition of resistance genes associated with different mobile genetic elements. In Gram-negative bacteria, many resistance genes are found as part of small mobile genetic elements called gene cassettes, generally found integrated into larger elements called integrons. Integrons carrying antibiotic resistance gene cassettes are often associate...
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I ntegrons, which are natural genetic engineering platforms, can incorporate open reading frames and convert them to functional genes by ensuring correct expression. Although such integrons play a major role in multidrug resistance phenomena among gram-negative species, similar platforms occur in numerous bacterial species, where they can play other roles. Meanwhile, we have evidence suggesting...
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Plasmids containing cloned integron fragments which differ only with respect to either the sequence of the promoter(s) or the number and order of inserted cassettes were used to examine the expression of resistance genes encoded in integron-associated gene cassettes. All transcripts detected commenced at the common promoter P(ant), and alterations in the sequence of P(ant) affected the level of...
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1.Eghtedarzadeh-Kondri, M., M.A. Walls and S.M. Glaser. 1997. Site-specific mutagenesis of immunoglobulin domains by multiple-fragment homologous recombination. BioTechniques 23:830-834. 2.Ho, S.N., H.D. Hunt, R.M. Horton, J.K. Pullen and L.R. Pease. 1989. Site-directed mutagenesis by overlap extension using the polymerase chain reaction. Gene 77:51-59. 3.Huff, J.P., B.J. Grant, C.A. Penning an...
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Campylobacter jejuni genetic manipulation is restricted by the limited number of antibiotic resistance cassettes available for use in this diarrheal pathogen. In this study, two antibiotic resistance cassettes were developed, encoding for hygromycin B and apramycin resistance, for use in mutagenesis or for selection of gene expression and complementation constructs in C. jejuni. First, the mark...
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ژورنال
عنوان ژورنال: Database
سال: 2011
ISSN: 1758-0463
DOI: 10.1093/database/bar054